Kudziwa Kuchuluka kwa Oxalic Acid mu Zipatso ndi Ndiwo Zamasamba Pogwiritsa Ntchito High-Performance Liquid Chromatography (HPLC)
1. Mfundo Yoyesera ndi Kapangidwe ka Njira
Monga asidi wamba wachilengedwe mu zipatso ndi ndiwo zamasamba, kuchuluka kwa oxalic acid kumakhudza mwachindunji kukoma ndi thanzi la chakudya. Kuyesera kumeneku kumagwiritsa ntchito reversed-phase high-performance liquid chromatography (RP-HPLC). Pansi pa acidic mobile phase conditions, oxalic acid imasiyanitsidwa ndi zinthu zosokoneza pogwiritsa ntchito column ya C18 chromatographic. Chowunikira cha ultraviolet chomwe chili pa 210 nm chimagwiritsidwa ntchito pofufuza kuchuluka, kutengera mawonekedwe a UV omwe amayamwa magulu a carboxyl m'mamolekyu a oxalic acid.
2. Mzere Wokhazikika ndi Kukonzekera Chitsanzo
Njira yochepetsera kuchuluka kwa madzi imagwiritsidwa ntchito pokonza njira zoyeretsera madzi: kuyeza molondola 25.0 mg ya oxalic acid dihydrate ndikuchepetsa mpaka 25 mL ndi madzi oyera kwambiri kuti mupeze yankho la 1 mg/mL. Izi zimachepetsedwa motsatizana mu mndandanda wa njira zoyeretsera madzi zomwe zili ndi 50, 100, 200, 400, ndi 800 µg/mL, ndipo kuchuluka kulikonse kumayikidwa mu katatu.
Pokonzekera zitsanzo, njira yochotsera pogwiritsa ntchito microwave imagwiritsidwa ntchito: yesani 5.00 g ya homogenate ya zipatso/masamba, onjezerani 10 mL ya yankho la 0.1 mol/L la hydrochloric acid, sakanizani pa 60°C ndi microwave kwa mphindi 10, sefani kudzera mu fyuluta ya nembanemba ya 0.45 µm, ndikusonkhanitsa fyuluta kuti muyesedwe.
3. Kukonza Mikhalidwe ya Chromatographic
Gawo loyenda ndi dongosolo la 0.01 mol/L potassium dihydrogen phosphate buffer (pH 2.5)–acetonitrile (95:5), lomwe limayenda ndi 0.8 mL/min ndipo kutentha kwa column ndi 35°C. Kusintha gawo la acetonitrile kumasonyeza kuti gawo lachilengedwe likapitirira 10%, nthawi yosungira ya oxalic acid imafupika kufika pa mphindi zosakwana 3, koma nthawi yomaliza yobwezeretsa imachitika. Pansi pa mikhalidwe yomaliza yokonzedwa bwino, nthawi yosungira ya oxalic acid ndi mphindi 4.2, zomwe zimapangitsa kuti pakhale kulekana kwathunthu ndi citric acid ndi malic acid oyandikana nawo (resolution > 1.5).
4. Kutsimikizira Njira
Kutsimikizira kwa mzere kumasonyeza ubale wabwino pakati pa malo okwera ndi kuchuluka kwa madzi pakati pa 10–1000 µg/mL (R² = 0.9993). Malire a kuzindikira (LOD), omwe amatsimikiziridwa ndi njira ya chiŵerengero cha chizindikiro-mpaka-phokoso, ndi 0.5 µg/mL. Mu mayeso obwerezabwereza, RSD ya ziwerengero zisanu ndi chimodzi zobwerezabwereza za chitsanzo chomwecho cha sipinachi ndi 1.8%. Kuyesa kubwezeretsa spike pamlingo atatu (80%, 100%, ndi 120%) kumapereka avareji ya kubwezeretsa kwa 98.2%, 102.4%, ndi 97.8%, motsatana, kukwaniritsa zofunikira pakusanthula kuchuluka.
5. Kusanthula Zitsanzo Zenizeni
Zipatso ndi ndiwo zamasamba zisanu ndi chimodzi zomwe zimapezeka m'masitolo zinayesedwa: sipinachi (356 ± 12 mg/100 g), udzu winawake (215 ± 9 mg/100 g), phwetekere (18 ± 2 mg/100 g), apulo (6 ± 1 mg/100 g), nthochi (sizinapezeke), ndi broccoli (89 ± 5 mg/100 g). Kuyerekeza ndi njira yadziko lonse (GB 5009.277-2016) kukuwonetsa kuti kusiyana pakati pa njira ziwirizi ndi kochepera 5%, kutsimikizira kudalirika kwa njira iyi. Makamaka, njira ya HPLC imawonetsa kukhudzidwa kwakukulu kuposa njira zachikhalidwe zowerengera pofufuza zitsanzo zochepa.
6. Mfundo Zofunika Kwambiri
Yang'anirani bwino pH panthawi yopereka chithandizo. Ngati pH ya chotsitsacho yapitirira 3, calcium oxalate imapangitsa kuti mayeso asakhale otsika. Gawo loyenda liyenera kukonzedwa mwatsopano tsiku lililonse kuti mupewe kutayikira kwa mchere ndi kutsekeka kwa mzati. Mukalandira jakisoni 20, tsukani mzati ndi acetonitrile-madzi (30:70) kwa mphindi 30 kuti mupewe kuwonongeka kwa ntchito ya mzati. Pa zitsanzo zokhala ndi utoto (monga kabichi wofiirira), tikulimbikitsidwa kuwonjezera njira yoyeretsera yochotsera mzati musanapereke jakisoni.
7. Malangizo Ogwiritsira Ntchito ndi Kukulitsa
Njira iyi ingagwiritsidwenso ntchito pozindikira oxalic acid m'zitsanzo zamoyo monga mkodzo ndi magazi. Mwa kusintha kapangidwe ka gawo loyenda (monga kuwonjezera tetrabutylammonium hydroxide), oxalic acid ndi metabolites zake zitha kudziwika nthawi imodzi. Kuphatikiza ndi chida chowunikira mass spectrometry, njira yolondola kwambiri yodziwira trace ingakhazikitsidwe. Mumakampani opanga chakudya, njira iyi ikhoza kupereka chithandizo chofunikira cha deta posankha mitundu ya oxalic acid yochepa komanso kukonza njira zophikira.
Zibo Anhao Chemical Co., Ltd. – Oxalic Acid
Zibo Anhao Chemical Co., Ltd. ndi kampani yodalirika yogulitsa komanso kutumiza kunja oxalic acid yapamwamba kwambiri (yomwe ndi ya mafakitale, 99.6% min) yochokera ku Shandong, China. Oxalic acid yathu ndi yoyera yolimba, yomwe imadziwikanso kuti ethanedioic acid, yokhala ndi formula ya molecular C₂H₂O₄. Ili ndi chiyero chapamwamba, zodetsa zochepa, komanso khalidwe lokhazikika m'magulu osiyanasiyana, ikukwaniritsa zofunikira za nsalu, zikopa, zitsulo, kukonza nthaka yosowa, kuyeretsa mankhwala, ndi mafakitale omanga.
Timapereka mankhwala a anhydrous ndi dihydrate oxalic acid, omwe amapezeka m'matumba a 25kg, matumba a 50kg, ndi matumba akuluakulu a 1000kg. Ndi njira zokhwima za QC, malipoti athunthu oyesera, komanso mapulani osinthika operekera, timaonetsetsa kuti zinthu zathu zikuyenda bwino komanso kuti ogula padziko lonse lapansi azigwiritsa ntchito bwino zinthu. Zogulitsa zathu zatumizidwa kwambiri ku Southeast Asia, Europe, Africa, ndi America, zomwe zadziwika kuti ndi zabwino komanso mitengo yopikisana.
Sankhani Zibo Anhao Chemical kuti mupeze oxalic acid yodalirika komanso mankhwala apadera.
Sankhani Zibo Anhao Chemical ya oxalic acid yoyera kwambiri yomwe imapereka magwiridwe antchito okhazikika komanso phindu ku bizinesi yanu. Lumikizanani nafe kuti mupeze maoda ambiri kapena zidziwitso zaukadaulo: info@anhaochemical.com
